What are the characteristics of HPLC?

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What are the characteristics of HPLC?

Characteristics Of High-Performance Liquid Chromatography

  • Fast analysis speed;
  • High separation efficiency;
  • High sensitivity;
  • Wide application range;
  • Columns can be used repeatedly;
  • Small sample size.

What are the advantages of HPLC?

The advantages of using HPLC for analysis are that it requires a small sample size, testing can be modified depending on level of quantification needed, and it produces reliable results.

What is the most important part of HPLC?

The infusion pump is one of the most important components of the HPLC system. Infusion pumps are classified into constant pressure pumps and constant flow pumps according to the factors of constant output liquid.

What are the characteristics of chromatography?

Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.

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What is the principle of HPLC?

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.

What are the different types of HPLC?

Different Types of HPLC Columns Used in Analysis

  • Normal Phase HPLC Columns: This type of columns has more polar stationary phase than the mobile phase.
  • Reverse Phase HPLC Columns:
  • Ion Exchange HPLC Columns:
  • Size Exclusion HPLC Columns:

What is the difference between HPLC and HPTLC?

HPLC is a chromatographic technique done through a column while HPTLC is planar. HPTLC is a planar chromatography were the solvent moves through a plate fixed stationary phase, due to capillarity. HPTLC is an improved version of TLC, while HPLC is a pump driven flow system with a stationary phase filled column.

What is the basic principle of HPLC?

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What are the principles of HPLC?

What is the purpose of HPLC?

The purpose high performance liquid chromatography (HPLC) analysis of any drugs is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease.

What type of chromatography is HPLC?

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).

What are types of HPLC?

Types of HPLC

  • Normal Phase HPLC. This method separates analytes on the basis of polarity.
  • Reverse Phase HPLC.
  • Size-exclusion HPLC.
  • Ion-Exchange HPLC.
  • Solvent Resorvoir.
  • Pump.
  • Sample Injector.
  • Columns.