Why only EDTA is used in complexometric titration?
Their main disadvantages are that they have low sensitivity and selectivity. Ethylenediaminetetraacetic acid (EDTA) complexometric titrations are based on the fact that many metallic ions form stable complexes with this tetradentate ligand, EDTA.
Why is standard EDTA solution standardized with standard hard water during hardness estimation?
Since [Metal ion]+ is unknown, you can make no measurement of the amount present, unless you know [EDTA] fairly accurately. And thus a known mass of primary standard is required to standardize, to calibrate the titration.
Why EDTA is not a primary standard?
Unfortunately EDTA cannot be easily used as a primary standard. The electron pairs of the carboxylic acid groups of EDTA are only available to the metal ion when the acid is dissociated, implying that the effectiveness of the complexing agent is maximized at high pH.
Why is EDTA not soluble in water?
The carboxyl groups of EDTA are not dissociated at low pH. Undissociated carboxyls (COOH) have no charge because the hydrogen is covalently bound and therefore acid EDTA is almost insoluble in water.
Is EDTA a secondary solution?
Unfortunately EDTA cannot be easily used as a primary standard. The H4Y form can be dried at 140◦C for 2 hrs and used as a primary standard, but is only sparingly soluble in water.
What is the principle of EDTA titration?
What is the principle of EDTA titration?.. An atom that has gained or lost electrons and thus has a net positive or negative charge is called an ion. EDTA is a compound that can form up to six bonds with a single metal ion, so it can bond with metal ions found in water.
Is EDTA a protease inhibitor?
As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. Bimake Protease Inhibitor Cocktail (EDTA free, mini-Tablet) is a blend of 5 pan-protease inhibitors for protection of protein integrity.
What is the theory of titration?
Titration is used for determining how much of an analyte in moles (or millilmoles) is in a solution. Analyte is the term for the dissolved unknown in the titration experiment. This is done by slowly adding a standard solution, or a reagent of known concentration, until the titration is determine to be complete.
What is complex titration?
complexometric titration (kəmˌplɛksəʊˈmɛtrɪk) n (Chemistry) chem a titration in which a coloured complex is formed, usually by the use of a chelating agent, such as EDTA , the end point being marked by a sharp decrease in the concentration of free metal ions.