Why is it important to find an enzyme that would cut the plasmid?
Why was it important to discard any enzymes that cut the plasmid at the replication site? (If the plasmid were cut at the replication site, it would not be able to reproduce and transfer genetic information to its host bacterial cell.)
Why is it important to use the same restriction enzyme to cut the plasmid and cut out the gene of interest?
Restriction enzymes cut at specific sequences so the same restriction enzyme must be used because it will produce fragments with the same complementary sticky ends, making it possible for bonds to form between them. Their sticky ends match, and so they can be ligated together.
What will happen if we use an enzyme that cuts at only one site?
They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites. When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.
Why is it important that the DNA is cut with the same restriction enzyme?
Restriction enzymes are useful for many different applications. Because the DNA sequence is different in each organism, the pattern of restriction sites will also be different. If DNA from a different organism is cut by the same restriction enzyme, a different set of fragments will be generated.
Why was it important to find an enzyme that would cut the plasmid at only one site what could happen if the plasmid was to cut at more than one site?
What could happen if the plasmid were cut at more than one site? You simply want to open the circular DNA so that the human DNA can be inserted into the circle. If the enzymes cut at multiple spots, then you would get multiple fragments.
Why might it be important to cut the DNA strand as?
Why might it be important to cut the DNA strand as closely to the desired gene as possible? Cutting the DNA close to the desired gene is necessan, so that undesired sequences are left out and the sticky ends find each other. The gene could also be used for study).
Why is it important that the same enzyme or enzymes be used to cut both the plasmid and the insulin gene from the human DNA?
Why is it important that the same enzyme or enzymes be used to cut both the plasmid and the insulin gene from the human DNA? it is important to use the same enzyme so that both the ends of the insulin and plasmid connect. Which antibiotic would you use to determine if the recombinant DNA was taken in? Kanamycin.
What is the importance of restriction enzymes?
The restriction enzyme prevents replication of the phage DNA by cutting it into many pieces. Restriction enzymes were named for their ability to restrict, or limit, the number of strains of bacteriophage that can infect a bacterium.
Why might it be important to cut the DNA strand as closely to the desired gene?
Why might it be important to cut the DNA strand as closely to the desired gene as possible? Cutting the DNA close to the desired gene is necessan, so that undesired sequences are left out and the sticky ends find each other.
Why do scientists use the same enzyme to remove the insulin and cut the plasmid open?
A bacterial plasmid is cut open using the same restriction enzyme . Restriction enzymes leave ‘sticky ends’, where one of the two DNA strands is longer than the other. Using the same restriction enzyme to cut both the human DNA and bacterial plasmid results in complementary sticky ends that join by base pairing.
Why was it important to discard any enzymes that cut the plasmid at the replication site and the antibiotic resistant gene site?
Why was it important to discard any enzymes that cut the plasmid at the replication site? If the replication site was cut, it would not function. The plasmid DNA waid cease to replicate in the cell thereby not multiplying for further study.
Why is it important to find an enzyme that would cut both the plasmid at one site and the human DNA at two sites?
Why was it important to find an enzyme that would cut the plasmid at only one site? If the enzymes cut at multiple spots, then you would get multiple fragments.