When would a Gram stain be used?
A Gram stain is most often used to find out if you have a bacterial infection. If you do, the test will show if your infection is Gram-positive or Gram-negative.
What are Giemsa stains used for?
Giemsa stain is performed on paraffin sections. It is used to stain the blood cells of hematopoietic tissues. It can also be applied to all tissue sections in which the presence of microorganisms is suspected. Gram + and Gram Bacteria are not differentiated with this staining.
What bacteria is detected using Giemsa stain?
Microorganisms such as Histoplasma, Leishmania, Toxoplasma, and Pneumocystis can also be detected with Giemsa, and in gastric tissues Helicobacter pylori (H. pylori) appear thin and distinctly blue.
What stains are used in Gram staining?
Reagents:
- Crystal violet (primary stain)
- Iodine solution/Gram’s Iodine (mordant that fixes crystal violet to cell wall)
- Decolorizer (e.g. ethanol)
- Safranin (secondary stain)
- Water (preferably in a squirt bottle)
What does epithelial cells in Gram stain mean?
Gram stains are usually scanned for the presence or absence of white blood cells (indicative of infection) and squamous epithelial cells (indicative of mucosal contamination).
How do you use Giemsa stain?
For Thin blood smear Stain with diluted Giemsa stain (1:20, vol/vol) for 20 min (For a 1:20 dilution, add 2 ml of stock Giemsa to 40 ml of buffered water in a Coplin jar). Wash by briefly dipping the slide in and out of a Coplin jar of buffered water (one or two dips). Note: Excessive washing will decolorize the film.
Why is the Gram stain so important?
Clinical Significance of Gram Staining The Gram stain is a very important preliminary step in the initial characterization and classification of bacteria. It is also a key procedure in the identification of bacteria based on staining characteristics, enabling the bacteria to be examined using a light microscope.
What is the purpose of primary stain in Gram staining?
The first reagent is called the primary stain. Its function is to impart its color to all cells. The second stain is a mordant used to in- tensify the color of the primary stain. In order to es- tablish a color contrast, the third reagent used is the decolorizing agent.
What are the uses or purpose of Gram staining specially in clinical setting?
Clinical Information The Gram stain is one of the simplest, least expensive, and most useful of the rapid methods used to identify and classify bacteria. The Gram stain is used to provide preliminary information concerning the type of organisms present directly from clinical specimens or from growth on culture plates.
How do you prepare a solution of Giemsa stain?
Giemsa stain preparation
- Dissolve 3.8g of Giemsa powder within 250ml of methanol.
- Heat the solution at 60oC.
- Add 250ml of glycerin.
- Filter the solution.
- leave the solution to stand for about 1-2 months before use. Store the solution in a cool, dark place.
What is the purpose of each solution used in Gram staining?
What is Giemsa stain used for in microbiology?
Giemsa stain is used for cytogenetics and histopathological diagnosis of parasites of malaria and other parasitic diseases. Giemsa stain can also be considered as a basic stain in classifying lymphomas in the classification of Kiel.
What is gigiemsa stain?
Giemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. It belongs to a group of stains known as Romanowsky stains.
What is the difference between eosin stain and Giemsa stain?
Eosin is an acidic dye that is attracted to the cytoplasm and cytoplasmic granules which are alkaline-producing red-orange coloration. Giemsa stain is specific for the phosphate groups of DNA. It attaches itself to regions of DNA with high amounts of adenine-thymine bonding.
How do you make Giemsa stain stock?
Preparation of the Giemsa Stain Stock solution (500ml) Into 250ml of methanol, add 3.8g of Giemsa powder and dissolve. Heat the solution up to ~60oC Then, add 250ml of glycerin to the solution, slowly.