What is the first thing you should do when using a pipette?
Steps to follow when using a micropipette
- Select the volume.
- Set the tip.
- Press and hold the plunger at the first stop.
- Place the tip in the liquid.
- Slowly release the plunger.
- Pause for a second and then move the tip.
- Insert the tip into the delivery vessel.
- Press the plunger to the second stop.
What are the do’s and dont’s in pipetting?
(1) When sucking the liquid, be sure to release the thumb slowly and smoothly. (4) Do not use a pipette to mix the liquid. (5) Do not use a large-scale pipette to remove a small volume of liquid to avoid affecting accuracy. Also, if you need to remove a larger amount of liquid over the volume, use a suction pipette.
How do you avoid contamination of samples or reagents in pipetting?
In daily laboratory work, pipette-to-sample contamination can be avoided by following these simple guidelines:
- Select a tip with the relevant purity class for your application.
- Use (sterilized) filter tips or positive displacement tips.
- Always change the pipette tip after each sample.
How do you avoid contamination of samples or reagent in pipetting?
Where do we use pipette?
Pipettes are generally used for moving small amounts of liquid or when measuring and dispensing liquid in mL units. When measuring less than 1 mL, Micropipettes are more accurate and user-friendly.
What do you use a pipette for?
To transport a measured volume of liquid
Pipette/Uses
How can we prevent contamination in laboratory samples?
However, there are measures you can take and protocols you can establish to reduce this risk and avoid contamination in lab samples.
- Automate the process.
- Wear proper protective equipment.
- Clean and sterilize equipment.
- Stay organized.
What are the safety precautions you must do to avoid contamination of the specimen?
After working a specimen with known or potential pathogens, immediately remove your gloves. Do not touch anything else in the room (e.g., the cryostats, staining rack, microscopes, telephones, etc.) with contaminated gloves. If you want to protect your hands from these surfaces, use a pair of clean gloves.
What precautions should be taken when performing manual pipetting?
It is not an excuse however, not to learn the proper precautions observed in manual pipetting. Here are important precautions involved in this procedure: * Before attempting to pipet anything, Personal Protective Equipment (PPE) should be worn at all times. This consists of a hair cap, a pair of gloves, a laboratory gown, a mask and goggles.
What is a pipette used for?
Safety Precautions for Using a Pipette | Sciencing The pipette (sometimes spelled pipet) is a useful piece of glassware still employed by many chemists. The function of a pipette is to use suction to draw up a set volume of liquid to allow it to be transferred to another container.
What are the dangers of using a pipette?
The danger is that you can misjudge the amount and draw up hazardous liquids into your mouth. Even if you don’t draw up the liquid, you can still inhale potentially harmful fumes. To work a glass pipette, you use a suction bulb to produce draw inside the tube.
Can I Use my Thumb to clean the side of pipette?
Do not use the thumb as it would be more difficult to manipulate. * Do not forget to wipe the sides of the pipette with a tissue or filter paper. This would remove excess liquid clinging to the external sides of the pipette, which may inaccurately add to the total volume of the solution.