What are the applications of HPLC?

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What are the applications of HPLC?

Applications of HPLC

  • Water purification.
  • Detection of impurities in pharmaceutical industries.
  • Pre-concentration of trace components.
  • Ligand-exchange chromatography.
  • Ion-exchange chromatography of proteins.
  • High-pH anion-exchange chromatography of carbohydrates and oligosaccharides.

What are the applications of chromatography technique in environmental monitoring?

Gas chromatography (GC) methods are used to analyze volatile and non-polar compounds. Liquid chromatography (LC) methods are employed in environmental analysis and monitoring for compounds such as those which are polar, non-volatile, thermolabile or compounds with a high molecular weight.

What are the different types of HPLC columns and their applications?

Different Types of HPLC Columns Used in Analysis

  • Normal Phase HPLC Columns: This type of columns has more polar stationary phase than the mobile phase.
  • Reverse Phase HPLC Columns:
  • Ion Exchange HPLC Columns:
  • Size Exclusion HPLC Columns:

What are the three applications of chromatography?

The applications of chromatography are :

  • It is used in DNA fingerprinting.
  • Used to quality analysis.
  • Used in food industries to analyse and separate the vitamins , preservatives etc .
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What are the three important application of chromatography?

It is used for the separation of different colors of ink. It is also used to identify and separate the preservatives and additives added in the food items. It is also used in DNA fingerprinting and bioinformatics. It is also used to separate different amino acids, proteins etc.

What are disadvantages of HPLC?

What Are the Disadvantages of HPLC?

  • Coelution. Because of the speed of HPLC and it reliance on different polarities of compounds, two compounds with similar structure and polarities can exit the chromatography apparatus at the same time or nearly the same time.
  • Adsorbed Compounds.
  • Cost.
  • Complexity.

What are the applications of HPLC in food industry?

Besides, the HPLC system is also connected to highly sensitive detectors like UV-visible and fluorescence spectrometers, electrochemical detectors, etc. 1. Clinical diagnosis of diseases, disorders. 2. In scientific research for discovery. 3. In pharmaceutical labs for analysis. 4. In the food industry for quality control. 5.

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What is the role of HPLC in environmental monitoring?

In case of Environment monitoring, HPLC is specifically used for water monitoring to detect pesticides presence. Environmental analysis in the US is regulated by the U. S. Environmental Protection Agency (EPA). They publish standard methods for analysis of many compounds in air, water, and soil.

What are the advantages of HPLC over other methods?

Then HPLC is much suited as it can analyze every molecule in the mixture. It finds its application to analyze and quantify the molecules. Components with similar chemistry and properties are easily distinguished by this method.

What are the applications of HPLC analysis in clinical diagnosis?

HPLC analysis in the clinical diagnosis and health industry: Many disorders related to body metabolism, those related to endocrine and exocrine gland secretion, alteration in body fluids are diagnosed by HPLC analysis of concerned fluids.