What do you mean by pBR322?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
Why is it called pBR322?
The vector pBR322 was named according to the standard rules for vector nomenclature. The “p” stands for plasmid. “BR” tells us which laboratory the vector was constructed in. They named the plasmid with the number “322” to distinguish this vector from other vectors they developed in their laboratory.
What is the full name of pBR322?
In PBR322 vector, the P is for the term plasmid and BR represents the scientist who invented this plasmid-“BOLIVAR”and”RODRIGUEZ” 322 STAND for the number assigned to segregate it from other types of plasmid.
What is pBR322 explain with diagram?
The pBR 322 vector is commonly used plasmid cloning vector in E. coli. In this, B and R stands for Bolivar and Rodriguez respectively. It is 4361 base pairs in length. Its molecular weight is 2.83 x 106 daltons.
Why is pBR322 used?
clone selection. Plasmid pBR322 is used extensively in genetic engineering. It has two genes of special interest. One codes for a protein that enables any host bacterium to resist the lethal effects of the antibiotic ampicillin and the other confers resistance to tetracycline.
How many recognition sites are there in pBR322?
pBR322 contains restriction sites for more than 40 restriction enzymes including BamHI, HindIII, SalI, PvuI, PvuII, PstI, EcoRI, ClaI. Further reading: Plasmid. What Is EcoR1?
What are the disadvantages of pBR322 vector?
Disadvantages:
- Conjugative.
- Detection scheme takes time.
- Selective pressure.
- Size of insert is only 6kb.
What is rDNA technology?
Recombinant DNA (rDNA) is a technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vehicles called vectors that ferry the DNA into a suitable host cell where it can be copied or expressed.
What is rDNA technology explain the steps?
There are six steps involved in rDNA technology. These are – isolating genetic material, restriction enzyme digestion, using PCR for amplification, ligation of DNA molecules, Inserting the recombinant DNA into a host, and isolation of recombinant cells.
Who prepared pBR322?
In pBR322 plasmid vector, – p – denotes that it is a plasmid; – BR – BR here stands for Boliver and Rodriguez who constructed this plasmid; – 322 – It is a number that was assigned to distinguish this plasmid from others developed in the same laboratory.
Which of the following sites are not present in pBR322?
pBR322 vector has restriction sites such as Hindlll, EcoRI, BamHI, Sall, Pvill, Pstl, Clal, ori (origin of replication). From the above discussion, we can conclude that pBR322 has a restriction site for Sall. And option ‘D’ says that Sall is not present in the pBR322 vector. So, our answer will be option ‘D’.
Why pBR322 is widely used?
This 4363-bp DNA molecule has been extensively used as a cloning vehicle because of its simplicity and the availability of its nucleotide sequence. Several vectors based on pBR322 have been constructed to overcome these limitations and to extend the vector’s versatility to accommodate special cloning purposes.
What is the size of pBR322?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 106 daltons.
What is the function of pbbr322?
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. The plasmid has unique restriction sites for more than forty restriction enzymes.
What is the history of the plasmid pBR322?
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after the postdoctoral researchers who constructed it.
What are the alternatives to pBR322 cloning?
An alternative to pBR322 cloning of DNA is to use pUC plasmids, which are modified pBR322 vectors with the ampicillin resistance gene and an added polylinker site similar to that in the μ13 mp vectors.