How do you calculate the specific activity of an enzyme?
In summary, specific activity = enzyme units / (vol. in µl x (protein conc. in mg per ml / 1000))
How do you calculate the specific activity of an enzyme Vmax?
The specific activity is Vmax/enzyme concentration. If the enzyme has only one substrate, then it is usually only necessary to measure the rate at a saturating substrate concentration, then divide by the enzyme concentration.
How do you calculate SDH specific activity?
Subtract the Sample Blank ∆A600 value from the Sample ∆A600 reading to obtain the corrected measurement. SDH activity is reported as nmole/min/μL = milliunit/μL. One unit of succinate dehydrogenase is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.
How do you find a specific activity from a total activity?
- total activity = (specific activity) x (total mg protein in preparation)
- \% yield – the amount of protein of interest retained in the purified fraction.
How do you calculate specific activity of radioactivity?
- Activity = λN.
- = (0.693/8 days) x (1/86,400 sec/day) x (3 x 1017 atoms)
- = 3 x 1011 atoms/sec I-131.
- = 3 x 1011 dps I-131.
How do you calculate specific activity from kcat?
kcat=Vm/[enzyme] if Vm is expressed in mM/s then [enzyme] has to be expressed in mM. So in your case if kcat=7.6/s then I can tell you that [enzyme] in the assay was 0.00043/7.6=0.0000566mM which is 56.6 nM.
What is MCAT specific activity?
A measurement of specific activity is used to describe the purity of an enzymatic mixture. The specific activity is equal to the activity of an enzyme per milligram of total protein in the mixture. Cooperativity is a unique type of allosteric regulation observed in proteins composed of multiple identical subunits.
What is enzyme activity and specific activity?
The enzyme activity refers to the number of moles of product formed per unit time. The specific activity is the ratio of the enzyme activity to enzyme concentration. This quantity can be used to measure the purity of our sample.
How do you measure specific activity?
Specific enzyme activity (usually stated simply as ‘specific activity’) is the number of enzyme units per ml divided by the concentration of protein in mg/ml. Specific activity values are therefore quoted as units/mg or nmol/min/mg (if unit definition B is applied).
How do you calculate enzyme concentration from enzyme activity?
How do you calculate specific activity of SDH?
How do you calculate total enzyme concentration?
The enzyme concentration in the reaction is 10 µg/ml = 10 mg/L = 0.01 g/L. The enzyme has a molecular mass of 50,000 g/mole, so we calculate the enzyme concentration to be (0.01 g/L) / (50,000 g/mole) = 2 x 10-7 mol/L = 0.2 µmoles/L.